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s100a4 antibody  (Bio-Techne corporation)


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    Structured Review

    Bio-Techne corporation s100a4 antibody
    S100a4 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb100-55404/S100A4+Antibody/bio-techne+corporation___nb100-55404
    Average 90 stars, based on 4 article reviews
    s100a4 antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Immunocytochemistry:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Immunofluorescence:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Blocking Assay:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Cell Culture:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Staining:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Concentration Assay:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali

    Diagnostic Assay:

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: on X-100 in 1× PBS for 5 min at RT. After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signali



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    Both mTORC1 and mTORC2 signaling are activated in kidney fibroblasts after IRI. a The graph showing the blood urea nitrogen (BUN) level in CD-1 mice at day 1 after UNx or IRI. *P < 0.05, n = 3 (left). Kidney histology as shown by periodic acid-Schiff (PAS) staining. Scale bar = 20 µm (right). b, c Western blot analyses showing the induction of p-Akt (Ser473), and p-S6 in the kidneys after ischemia-reperfusion injury (IRI). The numbers indicate each individual animal within the given group (b). The samples were pooled from three animals within each group (c). d Representative images showing the induction for p-Akt (Ser473) and p-S6 in Gli1- or <t>Fsp1-positive</t> fibroblasts from the IRI kidneys. White arrows indicate the co-staining positive cells. Scale bar = 20 µm
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    Both mTORC1 and mTORC2 signaling are activated in kidney fibroblasts after IRI. a The graph showing the blood urea nitrogen (BUN) level in CD-1 mice at day 1 after UNx or IRI. *P < 0.05, n = 3 (left). Kidney histology as shown by periodic acid-Schiff (PAS) staining. Scale bar = 20 µm (right). b, c Western blot analyses showing the induction of p-Akt (Ser473), and p-S6 in the kidneys after ischemia-reperfusion injury (IRI). The numbers indicate each individual animal within the given group (b). The samples were pooled from three animals within each group (c). d Representative images showing the induction for p-Akt (Ser473) and p-S6 in Gli1- or <t>Fsp1-positive</t> fibroblasts from the IRI kidneys. White arrows indicate the co-staining positive cells. Scale bar = 20 µm
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    Image Search Results


    Both mTORC1 and mTORC2 signaling are activated in kidney fibroblasts after IRI. a The graph showing the blood urea nitrogen (BUN) level in CD-1 mice at day 1 after UNx or IRI. *P < 0.05, n = 3 (left). Kidney histology as shown by periodic acid-Schiff (PAS) staining. Scale bar = 20 µm (right). b, c Western blot analyses showing the induction of p-Akt (Ser473), and p-S6 in the kidneys after ischemia-reperfusion injury (IRI). The numbers indicate each individual animal within the given group (b). The samples were pooled from three animals within each group (c). d Representative images showing the induction for p-Akt (Ser473) and p-S6 in Gli1- or Fsp1-positive fibroblasts from the IRI kidneys. White arrows indicate the co-staining positive cells. Scale bar = 20 µm

    Journal: Cell Death and Differentiation

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury

    doi: 10.1038/s41418-019-0336-3

    Figure Lengend Snippet: Both mTORC1 and mTORC2 signaling are activated in kidney fibroblasts after IRI. a The graph showing the blood urea nitrogen (BUN) level in CD-1 mice at day 1 after UNx or IRI. *P < 0.05, n = 3 (left). Kidney histology as shown by periodic acid-Schiff (PAS) staining. Scale bar = 20 µm (right). b, c Western blot analyses showing the induction of p-Akt (Ser473), and p-S6 in the kidneys after ischemia-reperfusion injury (IRI). The numbers indicate each individual animal within the given group (b). The samples were pooled from three animals within each group (c). d Representative images showing the induction for p-Akt (Ser473) and p-S6 in Gli1- or Fsp1-positive fibroblasts from the IRI kidneys. White arrows indicate the co-staining positive cells. Scale bar = 20 µm

    Article Snippet: After blocking with 2% normal donkey serum for 60 min, the slides were immunostained with antibodies against Gli1 (cat: 388516, RD Systems), Fsp1 (cat: NB100-55404, Novus), PDGFRβ (cat:14-1402, eBioscience), p-Akt (Ser473) (cat: 3868, Cell Signaling Technology), p-S6 (cat: 4858, Cell Signaling Technology), Rheb (cat: ab25873, abcam), Tsc1 (cat: 4906, Cell Signaling Technology), Rictor (cat: A300-459A, Bethyl Laboratories), cleaved caspase 3 (cat: 9664, Cell Signaling Technology), Ly6b (cat: MCA771G, AbD Serotec, Raleigh, NC), and CD3 (cat: 555273, BD Pharmingen), respectively.

    Techniques: Staining, Western Blot